Bio-Rad ChromLab™ Software - Manual (Page 131)

Priming and Purging the Systems
Instrument Guide | 129
To prime the buffer inlet valves
1. Immerse the tubing for the inlet valves in their respective buffer containers.
2. On the ChromLab computer or the touch screen, start ChromLab in Manual
mode.
3. In the System Pump dialog box, select the first inlet port to prime, for example
Buffer A 1.
4. Insert a 30 ml syringe into the priming port on system pump A.
5. Turn the priming port valve counter-clockwise one full turn to open the seal.
6. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and all the tubing is filled
with liquid.
7. Turn the priming port valve clockwise to close the port.