Detailed attributes – Bio-Rad ChromLab™ Software User Manual

Page 65

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Mixer

Instrument Guide

| 63

Detailed Attributes

The mixer is composed of a base motor, a stirring plate, and a hollow mixer barrel in

which the buffers are mixed. An optional mixer barrel extender is available to adapt

the mixer volume to the flow rate used. Bio-Rad offers several different mixer

chamber sizes for different flow rates. Table

2 and Table

3 list the recommended

barrel sizes for different flow rates for each type of system pump. To change the

barrels, see

Replacing the Mixer Components on page

186

.

Tip:

For liquids that are difficult to mix, use a mixer barrel with a higher

capacity.

Table 2.

Flow rates of supported mixer barrels for the F10 pumps

Mixer Barrel Size

Flow Rate Range, ml/min

Without buffer blending valve

(gradient)

With buffer blending valve

263 μl (base)

.01–1.00

0.1–1.99

750 μl

1.00–9.99

2.00–5.99

2 ml

10.00–20.00

6.00–11.99

5 ml

n/a

12.00–20.00

Table 3.

Flow rates of supported mixer barrels for the F100 pumps

Mixer Barrel Size

Flow Rate Range, ml/min

Without buffer blending valve

(gradient)

With buffer blending valve

750 μl (base)

1.00–4.99

<5.00

2 ml

5.00–14.99

5.00–11.99

5 ml

15.00–39.99

12.00–39.99

12 ml

40.00–100.00

40.00–80.00

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