Bio-Rad ChromLab™ Software - Manual (Page 72)

2 | The NGC Instrument
70 | NGC Chromatography Systems and ChromLab Software
How They Work
UV Detectors
ChromLab recognizes the connected optic module (that is, either the
single-wavelength UV or multi-wavelength UV/Vis module). The lamps turn on when
the NGC instrument powers up. At the start of the method run, the system
determines if the monitor is ready.
The warmup time for the LED lamp in the single-wavelength detector is negligible.
The tungsten and deuterium lamps in the multi-wavelength detector require 60–90
min to warm up.
Note: For most accurate data with the tungsten and deuterium lamps, allow a
minimum of 60 min warmup time.
Conductivity Monitor
The temperature-compensated conductivity monitor calculates the conductivity of
the sample and buffer mixture flowing through its flow cell. The stronger the salt
concentration, the higher the conductivity signal.
Since conductivity of a solution can vary with changes in temperature, temperature
variations of the sample are measured by the temperature sensor located in the
conductivity flow cell. A temperature compensation factor (set in ChromLab) can be
used to compare the conductivity of the sample in relation to the corresponding
conductance at room temperature or a user-defined reference temperature.