Bio-Rad ChromLab™ Software - Manual (Page 128)

3 | Preparing the Instrument
126 | NGC Chromatography Systems and ChromLab Software
Priming and Purging the NGC Discover System
Priming the NGC Discover system is a manual procedure and involves priming the
buffer blending valve starting from the Q4 port to Q1 then the buffer inlet valve
starting from buffer inlet A1. Purging the NGC Discover system is an automated
procedure and involves removing air from one or all buffer lines as well as the buffer
inlet valves and the system and sample pumps. Both procedures require access to
ChromLab.
Tip: You can perform the steps on the NGC touch screen.
On the NGC Discover system you prime and purge the following modules:
 Buffer blending valve module
 Buffer inlet valve modules
 Sample pump module
 Sample inlet module (NGC Discover Pro series)
Priming NGC Discover Systems
Note: This section assumes that you have not introduced buffer into the lines.
If you have buffer in the lines and are purging the lines of small air bubbles,
proceed to Purging the NGC Discover System on page 133 .
Important: T he instrument’s buffer lines cannot be primed when the system is
under pressure. If your system does not have a buffer blending valve, turn the
inject valve to Waste to release the system pressure before opening the priming
port.
To prime the buffer blending valve
1. Immerse the inlet tubing for ports Q1–Q4 in their respective buffer containers.
(See
Plumbing the NGC Discover System on page 278 for more information.)
2. On the ChromLab computer or the touch screen, start ChromLab in Manual
mode.
3. Set the fluidic scheme to NGC Discover.