Bio-Rad ChromLab™ Software - Manual (Page 164)

A | Maintaining the Instrument
162 | NGC Chromatography Systems and ChromLab Software
9. (Optional) Inspect and clean the piston.
Note: To replace the piston, you must remove the wash housing. For more
information, see Removing the Wash Housing on page 169 .
To clean the pump heads
1. Remove the pump head inlet and outlet check valves as explained in To remove
the pump head check valves on page 162 .
2. Immerse the entire pump head in an ultrasonic cleaner.
3. Sonicate the pump head with a standard cleaning solution for about 5 min, or
as necessary.
4. Rinse the pump head thoroughly with deionized water.
5. Reassemble the pump head. See Reassembling the Pumps on page 175 .
6. Insert the outlet lines into a waste bottle.
7. Run the pump at 1 ml/min with deionized water for about 15 min to ensure that
the seals are seated.
To remove the pump head check valves
1. Hold each pump head housing so its OUT check valve is on the top.
Tip: In this position, the IN check valve housing is on the bottom of the
pump head housing.