Ph detector – Bio-Rad ChromLab™ Software User Manual

Page 74

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| The NGC Instrument

72

| NGC Chromatography Systems and ChromLab Software

pH Detector

The pH detector includes an integrated flow cell in which to insert a pH electrode.

The pH of the solution flowing across the pH electrode is measured in real time. This

is very useful for applications in which knowledge of the buffer pH is critical for a

successful separation and/or the stability of the sample (for example, in antibody

purifications). The pH can be temperature compensated.

The pH valve directs the flow to either

pH — flow path is inline with the pH probe, or

Bypass — flow path bypasses the electrode

Note:

In Calibrate mode, the pH electrode is offline for calibration.

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