Bio-Rad ChromLab™ Software - Manual (Page 254)

B | Troubleshooting the Instrument
252 | NGC Chromatography Systems and ChromLab Software
Backpressure values
are higher or lower than
expected (continued). The inline filter is clogged. Inspect the inline filter and
replace if necessary.
Note: Recalibrate after
stopping the pumps. In
ChromLab, select Tools >
Calibrate > Pump Flow
Rate. Complete the
calibration steps in the
dialog box.
Unintended increase in flow rate. In ChromLab, verify that the
flow rate setting is correct.
Particulate matter has
accumulated in the system,
causing a resistance to flow.
Tip: Prevent or minimize
high backpressure by filtering
buffers and samples and by
changing the pump seals
before they deteriorate
completely.
Isolate the blockage in the
system tubing. Loosen the
connection fittings at the
detector and work backwards
toward the column and pump
head.
The rinse fluid is not
circulating. The tubing lines are not connected
or the fittings are not tight. Ensure that the wash system
tubing lines are connected
properly and reprime the
system.
The rinse fluid reservoir
is overflowing. Fluid is leaking past the pump
head seals into the wash system. Replace the pump head seal.
Symptom Possible Cause Possible Solution