Connecting the sample injection loops – Bio-Rad ChromLab™ Software User Manual
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Connecting the Sample Injection Loops
Instrument Guide
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Connecting the Sample Injection Loops
The NGC chromatography systems support a variety of injection loop types and
sizes, including static loops and dynamic loops. Table
supported loops.
Note:
You might have already connected a loop if you completed all of the
steps in the section Plumbing and Priming the NGC Chromatography Systems
in the NGC Chromatography Systems and ChromLab Software Installation
Guide.
To connect a loop to the sample inject valve
Connect one end of the loop to Loop E and the other end to Loop F on the
sample inject valve.
During Load, sample enters through Loop F while air, buffer, or excess sample is
pushed out through Loop E to waste.
During Injection
, buffer enters through Loop E and pushes the sample out to the
column through Loop F.