Bio-Rad ChromLab™ Software - Manual (Page 144)

3 | Preparing the Instrument
142 | NGC Chromatography Systems and ChromLab Software
Connecting Tubing to the Columns
Note: Ensure that the system has been purged of air and that the tubing is
filled with buffer before connecting the columns. Review and perform the tasks
in Priming and Purging the Systems beginning on page 118.
To connect tubing to the columns
1. Connect tubing from one of the following modules to the top of the column:
 Sample inject valve column port
 Column switching valve inlet port (1–5)
2. Remove the plug from the bottom of the column.
3. Connect the tubing from one of the following modules to the bottom of the
column:
 UV monitor
 Column switching valve outlet port (1–5)