Bio-Rad ChromLab™ Software - Manual (Page 266)

C | Plumbing the System
264 | NGC Chromatography Systems and ChromLab Software
Plumbing NGC Systems
Several plumbing tasks are required for all system configurations. Other tasks are
specific to the configuration. This section explains how to plumb each instrument
configuration.
General Guidelines for Tubing Sizes
The NGC systems use three sizes of tubing. Use the information in Table 18 to
select the appropriate tubing.
Note: When plumbing the system, keep tubing lengths to a minimum. Also, all
fittings should be finger tight. Do not overtighten, or you risk damaging the
connection. Bio-Rad offers tubing clips of different sizes in the accessories kit.
For outlet tubing longer than 6", attach a tubing clip in close proximity to the
fitting to keep tubing in place in the event that it detaches from the fitting.
Table 18. NGC system tubing sizes
Use Tubing Dimensions Tubing
Material Fittings Vol/cm
Prepump 1/8" OD x 0.062" ID
(3.2 mm OD x 1.6 mm ID)
Clear
PTFE
Super flangeless
for 1/8" (3.2 mm)
OD tubing 20 μl
Postpump
with F10
pump head 1/16" OD x 0.020" ID
(1.6 mm OD x 0.51 mm ID)
Orange
PEEK
Super flangeless
for 1/16" (1.6 mm)
OD tubing 2 μl
Postpump
with F100
pump head 1/16" OD x 0.030" ID
(1.6 mm OD x 0.76 mm ID)
Green
PEEK
Super flangeless
for 1/16" (1.6 mm)
OD tubing 4.5 μl