Bio-Rad ChromLab™ Software - Manual (Page 84)

2 | The NGC Instrument
82 | NGC Chromatography Systems and ChromLab Software
Air Sensor LEDs
The air sensor module is fitted with LEDs.
 Green LEDs indicate the module is connected.
 Blue LEDs indicate air in the tubing.
Prime the lines to remove air. See Priming and Purging the Systems on
page 118 for more information.
Air Sensor Tubing Length and Bubble Size
The response of the air sensor module is highly dependent on the bubble size and
flow rate, and requires a minimum tubing length between the sensor outlet and the
pump inlet. Table 8 lists recommended tubing lengths for various flow rates and
bubble sizes.
Table 8. Recommended air sensor tubing lengths
Flow Rate Bubble Size 1/8" ID FEP Tubing Length
(air sensor to pump inlet)
1 ml/min 15–25 μl ≥6"
5 ml/min 50–100 μl ≥6"
10 ml/min 200 μl ≥6"
100 ml/min 1.05–1.4 ml ≥42"