Bio-Rad ChromLab™ Software User Manual

Page 33

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Pumps

Instrument Guide

| 31

Each system pump module has an emergency Pause button and a Purge button.

Pressing the emergency Pause button on either system pump stops

both

pumps. If a run is in progress when you press Pause on either system

pump, both system pumps stop and the run pauses.

You can resume or cancel the run through ChromLab. If you choose to cancel

the run, you can save the run data up to that point.

Pressing Purge on a system pump runs that pump at full speed, replacing

any air in the lines with buffer. Purge activates purging for the tubing lines

that belong to that specific pump. For instance, when you press Purge on

system pump A, pump A runs at maximum flow rate while pump B

becomes idle. If you press Purge on pump B while pump A is purging, the

purge on pump A stops and the purge on pump B starts. The Purge button

is a toggle. To stop the purge, press Purge on that system pump again.

Note:

The Purge button is deactivated when:

A run is in progress

Any of the modules are in calibration mode

The buffer blending valve is included in the fluidic scheme

Caution:

To avoid damaging the column, always ensure that it is offline

when purging the system. Set the inject valve to waste, or remove the

column from the system and replace with a union fitting.

Tip:

You can pause the system through ChromLab using either the touch

screen or the computer running ChromLab (known in this document as the

ChromLab computer). You can also set timed purging of both pumps through

ChromLab, which will set the inject valve to Waste for a set amount of time. For

more information, see the

NGC Chromatography Systems and ChromLab

Software User Guide.

Pump Priming Port

The pump priming port introduces fluid into, and removes air from, the inlet tubing

line and the pump head.

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