Bio-Rad ChromLab™ Software - Manual (Page 297)

Using the C-96 Autosampler with NGC Systems
Instrument Guide | 295
Setting Up the C-96 Autosampler for Use in ChromLab
Methods
This section briefly explains how to set up the autosampler for use in a ChromLab
method. For information about creating methods in ChromLab, see the NGC
Chromatography Systems and ChromLab Software User Guide.
To set up a method that uses the autosampler
1. In ChromLab create a method that includes the autosampler in the fluidic
scheme. The Sample Application phase automatically displays Inject Using
Autosampler.
2. In the Sample Application phase, increase the injection volume to include the
total volume of the installed autosampler loop and the tubing needed to
connect Port 6 on the autosampler to the Loop F port on the sample inject
valve.
Tip: Typically, a sample loading volume of 2–3 ml should be sufficient to
completely load the entire sample in the autosampler loop onto the
column.
3. Use the front panel on the C-96 autosampler to create a method.
See the C-96 Autosampler for BioLogic DuoFlow™ Chromatography System
Quick Start Guide or the C-96 Autosampler for BioLogic DuoFlow
Chromatography System User Manual for more information.
4. Start the autosampler method and place the autosampler in REMOTE mode.
5. In ChromLab, complete the method setup and start the run.
For multiple samples, perform a multi-run of the ChromLab method. The number of
runs (in the multi-run) should correspond to the total number of injections (from the
same vial or multiple vials) to be programmed on the autosampler.