Bio-Rad ChromLab™ Software - Manual (Page 251)

System and Sample Pumps
Instrument Guide | 249
Liquid continuously
leaks from the pump
head washout drain
trough (continued). The check valves might need to
be replaced. Replace badly clogged or
damaged check valves.
Note: Recalibrate the
pumps after replacing
check valves. In
ChromLab, select Tools >
Calibrate > Pump Flow
Rate. Complete the
calibration steps in the
dialog box.
The screws that secure the pump
heads are not tight. Replace the wash housing small
seal.
Note: Recalibrate the
pumps after changing the
seals. In ChromLab, select
Tools > Calibrate > Pump
Flow Rate. Complete the
calibration steps in the
dialog box.
The backpressure
drops to zero or is
much lower than
expected. The flow rate setting might have
changed. Confirm that the flow rate
setting has not changed.
Tubing or fittings might be leaking. Inspect all tubing and fitting
connections, especially at the
pump inlet.
The pump seals might need to be
replaced.
Tip: A buildup of crystallized
buffer salts on the rear of the
pump head indicates that the
seals need to be replaced.
Replace pump seals.
See Disassembling the Pumps
on page 159 .
Symptom Possible Cause Possible Solution