Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 100)

Warning: Most USB portable memory devices display a flashing light when data is being
transferred. Wait until the light stops flashing, an indication that the file transfer is complete,
before removing the portable memory device from the instrument.
Note: An asterisk appears next to run data files that have been transferred to a USB
portable memory device.
9.1.2 Real-Time Data Transfer to Profinia Software
To transfer a run data file from the Profinia system to a computer in real time:
1. Before starting your purification run, use the USB cable provided with Profinia software
to connect the instrument (using the USB port on the back of the instrument) to a
computer that has Profinia software installed.
Important: To capture data in real time, the USB cable connection must be established
prior to starting a purification run.
2. Open Profinia software on the computer. A connection between the software and the
instrument will be indicated by a “Waiting for data” message at the bottom of the
Profinia software screen on your computer (Figure 9.2). This message indicates that a
run can be started.
Fig. 9.2. “Waiting for data” message on the bottom of the Profinia software screen.
3. Use the directory section of Profinia software in the upper left corner of the screen to
determine where you want your data file stored on your computer (see section 12 for
general information on running Profinia software).
4. Select Default from the software Options menu, and use Browse to select the file or
create a new file in the Profinia instrument for your run data information.
5. Touch the Start button on the Start Run screen.
Data from your purification displays in real time on your computer screen as the run
progresses.
9.2 System Utilities
The following list of calibration functions is accessible from the system menu on the Utilities
screen:
• Calibrate Touch Screen
• Pump Flow Rate Check
• Calibrate UV Monitors
• Calibrate Conductivity
• Adjust Peak Detection Sensitivity
• Calibrate pH Probe
• Calibrate Air Detection System
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