Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 191)

Appendix G
Profinia
™ System Annual Maintenance
The list of maintenance procedures in Table G should be performed regularly to ensure
optimum instrument performance and longevity.
Table G. Profinia system maintenance checklist.
Date
Maintenance Procedure Performed Comments
Examine the instrument to note any obvious
damage or spills.
Determine whether the instrument has been
malfunctioning, and note any problems
(if desired, use a Profinia starter kit to verify
operation).
Examine all external tubes (buffer inlet tubes,
sample inlet tubes, cartridge inlet and outlet
fittings) and replace any that are worn or
damaged.
Examine the diluent and waste bottles and
tubing; clean, repair, or replace as necessary.
Examine the cartridge connectors; replace
if worn or damaged.
Run the system ethanol wash and the
procedure to clean all lines and valves
(section 13.1.4).
Replace the inline filter disk.
Check UV monitor sample and reference
voltage (section 13.2.3). Replace UV lamp if
Vref is <0.2 V. Clean or replace UV flow cells if
Vsample is <65% of Vref.
Calibrate the pH probe (if installed), at pH 4 and
10 (section 9.6.2). Measure the pH of a pH 7
standard; replace the probe if the pH reading
is <6.9 or >7.1.
Check the pump flow rate (section 9.2.2) and
note the results.
Check the conductivity monitor calibration with
a standard solution, recalibrate if necessary
(section 9.2.4).
With instrument power off, remove the fluidics
compartment cover (section 13.2) to:
1. Correct any leaks and repair any damaged
tubing.
2. Replace the pump seals and the seals in
the piston guides (section 13.2.2).
3. Clean or replace the pump check valves
(two inlet, two outlet; section 13.2.1).
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