Bio-Rad Profinia™ Protein Purification Instrument User Manual

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Fig. 6.30. Start Run screen.

6.7.1 Viewing Steps

Touch the View Steps button (available on the Start Run screen at any point during the run)
to display the View Method Steps screen (Figure 6.31). During a run, the step that is
currently in progress is highlighted.

The following columns of information are displayed on the View Method Steps screen:

Step — numbered list of the primary steps that will be performed in the run

Name — brief description of the operation associated with the step

Conc. — buffer position in the instrument and buffer concentration (the instrument
dilutes concentrated buffers)

ml/min — flow rate displayed in ml/min

CV — column volume based on the cartridge size (for example, for a 1 ml IMAC cartridge,
2 CV = 2 ml)

Min — duration of each step in minutes

During a run, the step currently in progress is highlighted.

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