Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 69

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6.8.4 Canceling or Pausing a Run

To cancel the run (when you cancel a run, you proceed to the Prepare System to Clean
Lines screen):

1. Touch the Cancel Run button in the lower right of the screen. A confirmation screen

appears. The run continues until the cancel confirmation is made.

2. Touch OK to terminate the run, or Cancel to return to the Run in Progress screen and

continue the run.

To pause the run:

1. Touch the Pause button in the lower right of the screen to proceed to the Pause screen.

2. To resume the run, touch the Run button.

3. To cancel the run, touch the Cancel button.

6.8.5 Completing the Run

Run results display as soon as the end-of-run cleaning procedures are completed.

6.9 Preparing System for End-of-Run Cleaning

System cleaning is an automatic feature that occurs upon completion of each method run
and is required to prevent cross-contamination between runs and to maintain the system in
optimum condition. The two sample inlet lines and the fraction line valve (all eight fraction
lines) are cleaned as part of the end-of-run cleaning process. The pump seals are also
washed as the final step in this process and the internal system lines are stored with 20%
ethanol from buffer position 8 (Figure 6.38).

Fig. 6.38. Prepare System to Clean Lines screen.

6.9.1 Preparing for Line Cleaning

To prepare the system for end-of-run cleaning:

1. Remove all sample tubes from the instrument.

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