Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 104)

3. Fill the system’s water bottle with at least 500 ml of deionized water. Empty the waste
bottle if needed.
4. Touch the Data/Utilities button on the system’s home screen, then touch Calibration
Functions.
5. Touch the up and down arrows to scroll through the list of functions and highlight
“Calibrate Conductivity Monitor”, then touch Select to display the Calibrate Conductivity
screen.
6. Touch Next to proceed to the Calibrate Conductivity Flow Cell screen, which displays
calibration instructions.
7. Touch the Start button. The system pumps conductivity standard solution through the
conductivity flow cell, then set conductivity to 10 mS/cm. When finished with this step,
the system will pause and display “Calibration Complete”.
8. Place the tube of water in sample position 1, then touch the OK button. The system
flushes the sample line with water and when complete, returns to the Calibration
Functions screen.
9.2.5 Adjusting Peak Detection Sensitivity
Peak detection sensitivity settings range between 0.1 (most sensitive) and 10 (least
sensitive); the default setting is 1. Peak detection sensitivity is a global setting for both the
Bio-Rad methods and the program methods modes. The peak detection sensitivity parameter
allows the instrument to adjust the automatic peak detection algorithm to various peak
shapes and sizes in the event the default setting does not recognize a peak during the
specified time window. In general, use lower numbers (more sensitive) if a small protein
peak is expected.
Note: Using more sensitive settings for peak detection may lead to activation of automatic
peak detection from small deviations in the UV that are not associated with the protein
peak.
To adjust peak detection sensitivity settings:
1. Touch the Data/Utilities button on the system home screen, then touch Calibration
Functions.
2. Touch the up and down arrows to scroll through the list of functions and highlight
“Adjust Peak Detection Sensitivity”, then touch the Select button; the numeric keypad
displays.
3. Enter a value between 0.1 and 10, then press OK to return to the Calibration Functions
screen.
9.2.6 Calibrating pH Probe
The Profinia pH monitor is calibrated at pH 4 and pH 10. The following materials are
required to calibrate the pH probe:
• Standard pH calibration buffers — available from scientific supply companies;
approximately 50 ml of each buffer is required to calibrate the system. Using colorless
standards is recommended, since certain dye additives require additional wash steps
for removal
• Deionized water — diluent (water) bottle filled to 500 ml, and two sample tubes to 50 ml
to flush the system after calibration
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