Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 109)

Manual control toolbar — buttons (5) at the bottom of the Manual Operations screen
(Figure 9.6) that control:
• Toggle lamp — switches the UV lamp on or off: a light bulb icon in the upper left
corner of the screen indicates that the UV lamp is on. Normally the Profinia lamp
will turn off one hour after the last run is finished. If the lamp is manually switched
on, it will remain on until it is manually switched off
• Zero UV Detector 1 — sets the absorbance of UV monitor 1 to zero
• Zero UV Detector 2 — sets the absorbance of UV monitor 2 to zero
• Stop Pump — stops the system buffer pump, if running
• OK — returns to the Diag/Maint Functions screen
Fig. 9.6. Manual Operation of Hardware screen.
To access the manual operation features:
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
3. Touch the up and down arrows to scroll through the list of functions and highlight
“Manual Operation”, then touch the Select button.
The Manual Operation screen has three parts — the status screen, the manual operation
screen, and the toolbar (near the bottom of the screen):
Status screen — top of the Manual Operation screen, includes information regarding:
• PSI — system pressure value
• pH — value for pH. If pH probe is not connected the value shown is ~25
• T — system temperature value
• AU1 and AU2 — absorbance values for UV monitor 1 and UV monitor 2
• VR1 and VR2 — lamp operation indicator; must be greater than 0.05 volts
• VS1 and VS2 — flow cell operation indicator; must be greater then 65% of the VR
value
• C — conductivity value in mS
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