Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 51)

Note: Some kits do not require a reagent bottle in every position. If a position does not
require a bottle (indicated by an “X” on the installation screen), leave that position empty, or
place a bottle with water in that position.
6.5.2 Installing Sample Tubes
The sample loading area is on the front right side of the instrument (Figure 6.16).
Fig. 6.16. Instrument sample loading area.
To load sample(s):
1. Add the required amount of sample to the sample tubes as indicated on the touch
screen. If you are running more than one sample, place sample 1 in the sample 1 position,
and sample 2 in the sample 2 position (S1 and S2 on the installation screen, respectively).
Warning: If end of sample detection has been disabled, enter a sample volume approximately
0.5 ml less than the actual sample volume to prevent air from entering the instrument (see
Section 9.3.12 for more information).
2. Place sample lids on the sample tubes.
3. For each sample tube position, lift the sipper tube away from the instrument and insert
the sample tube into the sample sipper tube. If only one sample is needed (S1), the
second sample position (S2) can remain empty, or place a tube with water in the S2
position.
44
Sample lid