Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 161)

the UV monitor cuvette. If the reference voltage is less than 0.05 volts, the lamp intensity is
low and the lamp should be replaced. If the sample voltage is less than 65% of the reference
voltage, the flow cell may be dirty. Either of these conditions may cause an unstable UV
baseline.
To check voltage:
1. Switch on power to the Profinia instrument.
2. From the system home screen, touch the Data/Utilities button.
3. Touch the Diag/Maint Functions button.
4. Select the Manual Operation button.
5. Touch the “Toggle Lamp” button, at the bottom of the screen. The “lamp” icon should
appear at the upper left hand corner of the display.
6. Wait 10 minutes for the lamp to warm up.
7. The Sample and Reference values will be displayed in the upper left corner of the
Manual Operation of hardware screen.
Replacing the UV Monitor Lamp
Safety warnings:
• Do not remove the UV lamp when the Profinia instrument is turned on; the UV light can
injure your eyes or skin
• The UV lamp gets hot during operation. Allow 15 min to cool before touching the lamp
assembly to avoid burns
• Do not touch the glass surface of the lamp. If this happens, clean with methanol and a
clean cloth and allow it to dry before installing
• The lamp contains a small amount of mercury vapor — dispose of the lamp in accordance
with local regulations. Do not break or incinerate the lamp
The Profinia instrument’s UV lamp is located inside the instrument cabinet. The Profinia UV
lamp replacement kit (catalog #620-0406) is required to replace the lamp. To replace the
Profinia UV lamp:
1. Use the procedure in section 13.2 to remove the instrument access panel.
2. Locate the UV monitor assembly (Figure 13.13).
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