Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 105)

To calibrate the pH probe:
1. Touch the Data/Utilities button on the system home screen, then touch Calibration
Functions.
2. Touch the up and down arrows to scroll through the list of functions and highlight
“Calibrate pH Probe”, then touch the Select button. An information screen appears.
3. Touch the Next button; the Calibrate pH Probe screen displays with a list of detailed
instructions.
4. Pour 50 ml of each pH buffer (pH 4 and pH 10) in separate 50 ml sample tubes.
5. Place the bottle containing the pH 4 buffer in sample position 1.
6. Place the bottle containing the pH 10 buffer in sample position 2.
7. Touch the Start button. The system will:
a) Flush the pH probe with water.
b) Pump pH 4 buffer to prime sample line 1 and the pH probe. The system reduces
the flow rate and sets the pH value to 4.
c) Pump pH 10 buffer to prime sample line 2 and the pH probe. The system reduces
the flow rate and sets the pH value to 10. The system pauses and displays “pH
Probe Calibration Complete”.
8. Replace the tubes of pH buffer (sample positions 1 and 2) with 50 ml tubes filled with
deionized water.
9. Touch the Start button. The system flushes the lines with water, then returns to the
Calibration Functions screen.
9.2.7 Calibrating Air Detection System
Calibration of the three air sensors is required for optimum performance. The calibration
process measures electronic signals of air and liquid to establish the thresholds for end of
sample/reagent detection. To calibrate the air detection system, follow the on screen
instructions.
9.2.8 Calibrating Pressure Sensor
The Pressure Sensor Calibration process contains the re-calibration for the zero point of
the pressure measuring system. Calibration of the zero pressure point is needed if the
pressure reading during non-operation of the system does not return to zero. Access the
Manual mode from the Data/Utilities section to observe the pressure reading.
To calibrate the zero pressure point, access the Calibrating Pressure Transducer from
Home/Data-Utilities/Calibration Functions and follow the on-screen directions.
9.3 Diagnostic/Maintenance Functions
The following list of diagnostic and maintenance functions is accessible from the system
menu on the Diag/Maint Functions screen (Figure 9.4):
• Display Firmware Version
• Manual Operation
• Set Time and Date
• Clean Sample Lines
• Clean Fraction Lines
• Clean Buffer Lines
• System Ethanol Wash
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