Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 91)

7.8.1 Viewing Steps
Touch the View Steps button (available on the Start Run screen, and at any point during the
run) to retrieve the View Method Steps screen. During a run, the step that is currently in
progress is highlighted.
7.8.2 Running the Manual Prime Function
Note: The manual prime function is needed to prime any extra tubing up to the point of
connection to original inlets.
To prime extra tubing attached to buffer, sample, fraction tube, diluent, or waste outlets to
accommodate larger containers, touch the Manually Prime button at the bottom of the Start
Run screen. The Select Item to Prime Manually screen displays (Figure 7.14).
Fig. 7.14. Select Item to Prime Manually screen.
1. Touch the arrow buttons to scroll through the list of reagent lines and highlight the line
that requires manual priming.
2. Touch the Prime button to start priming. The pump continues priming until you touch
the Stop button.
3. Select the next reagent line for manual priming (if needed), and press Prime.
4. Continue until all reagent lines connected with extra tubing have been primed.
Note: Prime lines until no air is visible in the tubing or when fluid is seen entering the waste
container.
Priming Multiple Lines
To prime multiple lines, either follow the procedure outlined in the previous section, or:
1. Touch the arrow buttons to scroll through the list of reagent lines, highlight the desired
line, then touch the Prime button.
2. While the system is priming the first reagent line, use the arrow buttons to select the
next reagent line (priming of the first line continues).
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