Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 159)

12. Press the new seals into the recesses in the pump body with your fingers, then use the
piston seal tool to snap them into place. Soak the pump body in 50% alcohol, sonicate
in alcohol if possible to remove any trapped air.
13. To replace the piston carrier seals, pull the pistons from the piston carriers, and wash
all parts with deionized water.
Fig. 13.10. Piston and piston carrier.
14. Use the piston seal tool to remove both piston carrier seals from the piston guide by
inserting the tip of the tool in the seal and gently prying sideways until the seal pops
loose from the recess (Figure 13.11).
Note: Each piston guide has seals at one end; these are identical to the main seals.
Fig. 13.11. Removing the piston from the piston carrier (left); and removing the piston carrier seal, using
the piston seal tool (right).
15. Rinse the piston carrier in water, and press the new seal into place with your finger. Do
not use the seal tool to install the new seal.
16. Reassemble the pistons, springs, and carriers.
17. Push the piston carriers into the pump head recess.
Note: The flats on the piston carriers must be straight up and down (Figure 13.12).
16. Wipe off the four screws and place a small amount of Vaseline or stopcock grease on
the last 1/8" of the screws. Reconnect the piston wash tubing to the top of the pump
head (2 fittings).
17. Place the screws in the pump head, and put the pump head back in the hole in the
carrier. If you have trouble getting the pump head inserted, check to see that the flats
on the guides align with the flats on the carrier assembly (Figure 13.12).
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