Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 209

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Appendix K
Profinia Program Methods Planning Worksheets

The worksheets included in this section are designed to help plan desired changes to step
parameters when customizing a program method. The worksheets provide an overview of
all main method steps and parameters associated with the steps; parameters that are not
editable are indicated by an “X” in the corresponding position. Worksheets are included for
the following method types:

•

Native IMAC only

•

Native IMAC plus desalting

•

Denaturing IMAC

•

GST only

•

GST plus desalting

•

Protein A and G only

•

Protein A and G plus desalting

•

Profinity eXact

•

Profinity eXact plus desalting

•

Affinity only

•

Affinity plus desalting

•

Desalting only

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