Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 156)

Note: Do not overtighten the fittings.
Finishing Pump Check Valve Maintenance Procedures
When finished with check valve cleaning or replacement procedures:
1. Replace the access panel. It is best to thread all four thumbscrews loosely into the
instrument body, then tighten.
2. Reconnect the power cord and tubing.
3. Replace the waste bottle and reconnect the waste tube to the instrument.
4. Turn the unit around so the front is facing outward.
13.2.2 Pump Seals
The Profinia system automatically cleans the flow path after every run, including the area
behind the pump seals. The Profinia pump has four seals, two main seals located in the
pump body, and two piston guide seals located in the piston guides. Pump seals do not
normally require replacement, except during periodic preventative maintenance. If flow
rates are not as expected or sample volumes are too small, the pump seals may be leaking.
The Profinia pump seals are located in the instrument cabinet, inside the pump assembly.
Materials required to clean or replace the pump seals:
• Pump seal kit — catalog #620-0412, includes four pump seals, pump seal tool, and
instruction sheet
• 9/64" Allen wrench
• Wrench for tubing fittings
• Profinia 125 ml bottle — filled with 100 ml of 50% alcohol (methanol, ethanol, or
isopropanol)
• Shallow tray and alcohol — for soaking pump parts
To clean or replace the system pump seals:
1. Use the procedure in section 13.2 to remove the instrument access panel.
2. Locate the pump head assembly.
3. Use your fingers or the tubing wrench to remove the 1/8" inlet tube from the front of the
pump body (Figure 13.6).
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