Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 168)

14. Turn on the unit and wait 5 min.
15. Check the sample and reference voltages, as described above.
13.2.5 Buffer and Sample Inlet Tubes
If the buffer or sample inlet tubes become worn or damaged, replace them with 1/8" OD
Tefzel tubing. Precut pieces of tubing are available (catalog #620-0405, Profinia sipper tube
replacement kit). You may also use tubing from a roll. Cut this tubing to 13.46 cm (5.3")
long, and straighten it; heat it with a hair dryer if straightening is difficult. Pull the existing
tubing from the silicone tubing coupler and replace with the new tubing.
13.2.6 Tubings and Fittings
Under normal circumstances, the internal tubing and fittings for the Profinia system should
last indefinitely; they are protected from damage by the instrument’s cabinet and the system’s
automatic cleaning functions. Should the tubing require replacement, however, you may
order the Profinia instrument tubing kit (catalog #620-0415), which contains tubing and
fittings. It is important that the replacement tubing is the same diameter as the original, and
the length must be within ±3 mm, because method programming is based on the volume of
the tubing.
You may also make a replacement tube from bulk tubing and fittings. Tubing fittings are
¼-28 flat bottom, using lock rings and ferrules to make a seal. The small tubing is 1/16"
Tefzel (Bio-Rad catalog #750-0602). Fittings for the 1/16" tubing are nut/ferrule/lock ring
sets (catalog #750-0554). The large tubing is 1/8" Tefzel (Bio-Rad catalog #750-0603).
Fittings for the 1/8" tubing are nut/ferrule/lock ring sets (catalog #750-0553).
To make a replacement tube:
Note where each end of the tubing connects (Table 13.1). Refer to the diagram on the
inside of the fluidic access panel or to the diagram below (Figure 13.24) to find the
connection inlets and outlets.
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