Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 203)

Table 2. Recommended Formulations for Buffers and Solutions for 1 ml eXact + 10 ml
Desalting Method
*These buffers and solutions are available as part of the Profinia Desalting Kit (catalog # 620-0228) or individually as concentrates.
The buffers will need to be manually diluted to 1x for use with the default eXact method, or a custom method can be created in the
Program Methods mode to accommodate the concentrates, if desired.
†Volumes used for the 5 ml eXact methods will be correspondingly larger to accommodate the increase in cartridge capacity and size
Table 3. Triggering Anions for the Profinity eXact Tag System
Anion Compound
Fast
Cleavage
Moderate
Cleavage
F NaF, KF 100 mM 5 mM
N
3
NaN
3
10 mM 1 mM
NO
2
NaNO
2
5 mM 1 mM
HCO
2
NaHCO
2
1000 mM 25 mM
Cl NaCl, KCl >1000 mM 75 mM
-
-
-
-
-
Buffer Conc. [x] Formulation Volume†
Port
# Catalog #
eXact Bind/Equilibration/Wash Buffer 1x 100 mM Sodium Phosphate, pH 7.2 65 ml B1 -
eXact Wash Buffer 2 (Optional) 1x
100 mM Sodium Phosphate, 300 mM
Sodium Acetate, pH 7.2
35 ml B2 -
eXact Elution Buffer (Recommended) 1x
100 mM Sodium Phosphate, 100 mM
Sodium Fluoride, pH 7.2
40 ml B3 -
eXact Elution (Optional) 1x
100 mM Sodium Phosphate, 20 mM
Sodium Azide, pH 7.2
40 ml B3 -
Profinia Desalting Buffer (PBS)* 1x
137 mM NaCl, 2.7 mM KCl, 4.3 mM
Na
2
HP0
4
, 8.1 mM KH
2
P0
4
, pH 7.4 75 ml B4 620-0216
Cleaning Solution 1* 1x 500 mM NaCl, 50 mM Tris, pH 8.0 40 ml B5 620-0217
eXact Regeneration Solution 1x 100 mM Phosphoric Acid 55 ml B6 -
eXact Storage Solution 1x
100 mM Sodium Phosphate
0.02% Sodium Azide, pH 7.2 60 ml B7 -
20% EtOH (system storage solution) 1x 20% EtOH (v/v) 35 ml B8 -
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