Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 83)

Most steps show the following information and parameters:
• Method name — editable in the Enter Run & Sample Information screen
• Step name — cannot be edited
• Flow rate and column volume — limited parameters; dependent on method type,
cartridge type and size, and operating temperature (for desalting cartridges only).
The numeric keypad displays the value limits immediately below the text line if the
entered values exceed the limits
• Step time — calculated from the entered values for flow rate and column volume;
cannot be edited
• Concentration — buffer position in the instrument and the concentration of that
buffer. The instrument dilutes the concentrated buffer to 1x. Concentration values
allowed are whole integers between 1 and 5
• Frac — fraction number indicates the fraction designation for that step and cannot
be edited
4. Touch the arrow button to scroll through step parameters. The Edit button is available
only for those parameters that can be edited.
5. Highlight the parameter you want to edit and touch the Edit button to retrieve the
numeric keypad.
6. Enter new values for the parameter in the text line.
7. In the numeric keypad, touch the OK button to accept the new value or Cancel to revert
to the previous value. You will return to the Select Step Parameter to Edit screen.
8. When finished editing parameters for the step, touch the OK button to return to the
Method Information screen.
9. Repeat this procedure to edit all desired step parameters for your method, then touch
the Save button to store the method in memory. When the method is saved in the
Method Information screen it will be saved with the name as shown in that screen. If a
different name is needed, return to the Enter Run & Sample Information screen and edit
the method name.
10. Touch the OK button at the bottom of the Method Information screen to return to the
Enter Run & Sample Information screen.
Editing Peak Detection Parameters
Peak detection parameters are available for the protein elution steps when the bound
protein is eluted from either the affinity cartridge to the desalting cartridge or directly from
the affinity or desalting cartridge to a fraction tube.
76