Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 80)

• Volume — user-entered sample volume between 2 and 999 ml. If you have chosen to
perform a run with two samples, a volume must be entered for each sample. If the
sample volume is greater than 50 ml, the instrument sample line(s) can be connected
directly to an external sample container (see section 11.2 for details on external
connections). Make sure to follow the manual priming protocol at the Start Run screen if
using external containers. See Appendix E for sample preparation details
• A
280
of 1 mg/ml — extinction coefficient for the protein is expressed as the absorbance
of a 1 mg/ml solution of the protein of interest at 280 nm for a 1 cm UV pathlength.
The default value for this parameter is 1.00. An extinction coefficient can be assigned
for each sample. This value is used to calculate the estimated total protein and protein
concentration at the end of the run
• Home — returns to the home screen. All entered run and sample information, except
lot number tracking information, will be cleared when you touch the Home button. The
entered lot number tracking information remains in temporary memory as long as
instrument power is on. When cycling power, the lot number tracking information
defaults to “BLANK”
7.3.1 Adding or Editing Run and Sample Information
To add or edit sample and run information:
1. Touch the arrow buttons to the right of the information text box to scroll through the list
of options and highlight the parameter for which you’d like to add information.
2. Touch the Edit button to access the alphanumeric or numeric keypad. The appropriate
keypad displays.
3. Enter or edit information for the selected parameter in the text line at the top of the
screen.
Note: If values entered exceed the system-set limits, the value limits appear immediately
below the text line of the numeric keypad and the system overrides the entered value with
the maximum or minimum value allowed.
4. Touch OK to accept all changes or Cancel to delete any entered data and return to the
Enter Run & Sample Information screen.
5. Repeat this process for all information parameters. For details on entering lot number
tracking, see section 7.3.2 below.
6. The Save button displays when the method name and method parameters have been
edited. Touch the Save button to save the method in memory.
Note: Editing the name of a saved method file does not delete the original file. If the
method with the edited name is saved, both files will be accessible in the list of saved
methods (see section 8 for information on retrieving saved methods and renaming existing
methods).
7.3.2 Adding Lot Number Tracking Information
To add lot number information for any of the reagents or components used in your method
run:
1. Touch the arrow buttons to scroll through the list of options and highlight “Lot # Tracking”.
2. Touch the Edit button. You will proceed to the Edit Lot Number Information screen.
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