Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 60)

Fig. 6.27. Install Bio-Scale Mini Cartridges screen showing sample loop installation in the cartridge 1
position.
To connect and fill the desalting loop:
1. Insert the male luer fitting from cartridge holder 1 into the top female luer fitting of the
3-way stopcock. Ensure that the second female luer fitting of the 3-way stopcock is
facing out so the syringe may be easily inserted.
2. Connect the male luer of the end of the desalting loop to the female luer fitting of
cartridge holder 1.
3. Install the desalting cartridge in the cartridge 2 position. Touch the Next button to proceed
to the Start Run screen (Figure 6.28).
Fig. 6.28. Start Run screen directs application of the sample to the sample loop (desalting-only method).
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