Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 48)

• Fraction collection tubes — indicated on the touch screen by a set of four tubes on
the left labeled 1A through 1D for sample 1 collection, and four tubes on the right
labeled 2A through 2D for sample 2 collection. Fraction tubes are placed at the bottom
of the instrument. Each fraction tube shows the volume that will be collected in the tube.
Only the required fraction tubes are represented on the Install Reagent, Sample &
Fraction Tubes screen. Before beginning your run, make sure the fraction tube capacities
match the volumes indicated on the screen
Note: The fraction positions are numbered 1A through 1D from left to right, and 2A through
2D are ordered right to left. This places the purified protein in the center two positions for
easiest access and for use with the cooling accessory.
• View Details — lower toolbar button; proceeds to a screen that displays the name for
each of the components shown in the system setup screen (Figure 6.11). Touch OK to
return to the Install Reagents, Sample & Fraction Tubes screen
Fig. 6.11. Review Buffer & Fraction Details screen displayed when the View Details button is selected.
6.5.1 Loading Buffers
The Bio-Rad Methods function of the Profinia system is designed to operate with Profinia
purification kits that contain concentrated buffers and cartridges for each preprogrammed
method type. To prepare reagents for the method run:
Note: See Appendix I and J for recommended reagents for the Protein A and G and
Profinity eXact methods.
1. Remove reagents from the Profinia purification kit designed for your selected method
type (see section 10).
2. Prepare reagents as specified in the purification kit instruction manual. Some of the
buffers require the addition of dry reagents followed by filtration, such as those for
denaturing IMAC and GST methods.
3. When reagents are ready for loading into the Profinia instrument, place a buffer lid on
each buffer bottle (Figure 6.12).
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