Bio-Rad Profinia™ Protein Purification Instrument User Manual
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Section 3
Key Terms and Definitions
3.1 Bio-Rad Methods
The following automated methods — Bio-Rad methods — for common affinity-tagged
protein purification and desalting procedures have been programmed into the Profinia
™
protein purification system:
•
Native IMAC* — native conditions with His-tagged proteins; use the Profinia native
IMAC purification kit
•
Native IMAC and Desalting — native conditions with His-tagged proteins, followed by
desalting before elution to the fraction tube; use the Profinia native IMAC purification kit
•
Denaturing IMAC — denaturing conditions with His-tagged proteins; use the Profinia
denaturing IMAC purification kit
•
GST** — native conditions with GST-tagged proteins; use the Profinia GST purification
kit
•
GST and Desalting — native conditions with GST-tagged proteins, followed by desalting
before elution to the fraction tube; use the Profinia GST purification kit
•
Protein A and G — affinity purification of antibodies (typically IgG) from serum, ascites,
or hybridoma cell culture supernatant using Protein A (or in some cases, Protein G)
chromatography media
•
Protein A and G and Desalting — affinity purification of antibodies (typically IgG) from
serum, ascites, or hybridoma cell culture supernatant using Protein A (or in some
cases, Protein G) chromatography media followed by desalting
•
Profinity eXact — affinity purification with on column tag removal for eXact affinity
tagged proteins
•
Profinity eXact and Desalting — affinity purification with on column tag removal for
eXact affinity tagged proteins followed by desalting
•
Desalting — buffer exchange and desalting of a protein solution; use the Profinia
desalting kit and desalting sample loop accessory
* IMAC = immobilized metal affinity chromatography.
** GST = glutathione S-transferase.
3.2 Program Methods
Program methods can be customized using the Bio-Rad methods as templates. Method-step
parameters, such as flow rates, column volumes, and automatic peak detection settings can
be edited to optimize sample-specific purification conditions. Each program method can be
saved with the generic template name or can be given a unique name. Up to 35 program
methods can be saved in memory for future retrieval and use.
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