Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 173)

13.2.7 Inline Filter
The inline filter is a 6 µm polyester membrane filter, which protects the system from particles
in the inlet fluid stream. The inline filter is located under the top cover of the instrument
above the buffer bottles, near the left side of the instrument. The inline filter should be
replaced every month, or after 20 purification runs.
Preparing the filter for installation:
1. Fill the Luer-Lok syringe with 30 ml of 20% ethanol and connect to the inline filter
(Figure 13.27).
2. Push the ethanol through the inline filter in approximately 30 sec.
3. Remove the inline filter from the syringe and follow installation instructions.
Fig. 13.27. Connecting the syringe to the inline filter.
Installing the filter:
1. Open the top cover and locate the filter (Figure 13.28).
2. Twist both luer fittings counterclockwise to unlock them, then remove the filter.
3. Install the new filter, and tighten the fittings.
Fig. 13.28. Location of the inline filter under the instrument top cover.
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