Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 65)

Once memory has cleared, the method starts and the Run in Progress screen displays.
6.8 Monitoring Run Progress
6.8.1 Run in Progress Screen
The Run in Progress screen (Figure 6.35) displays an overview of the current run, as well
as basic details about the current step in progress and expected sample elution times.
Fig. 6.35. Run in Progress screen.
Note: The View Steps screen can be accessed from both the Start Run screen and the
Run in Progress screen at any time while the run is in progress. To return to the Run in
Progress screen, touch the OK button.
The Run in Progress screen displays the following information:
• Elapsed/total run time — elapsed time versus the total run time for the method. The
total run time indicated refers to the time necessary to complete the protein purification
and cartridge cleaning
• Progress bar — percent of total run time that has elapsed. The arrows underneath the
progress bar indicate the point at which the sample will be eluted. If you have chosen to
perform a run with one sample, there will be one arrow linked to an S1 symbol; for
two-sample procedures, there will be S1 and S2 arrow indicators
• Current step — name of the current step in progress
• Step time remaining — duration of time left to complete the current step in progress
• Expected elution times — time at which each programmed sample is expected to
elute. As soon as the protein is eluted, the actual time of elution and estimated protein
yield and concentration are displayed
Note: Make sure the time and date are properly set in the Utilities menu. The sample elution
time is shown in system clock time as set by the user (see section 2.2.3 or 9.3.3 for
instructions on setting the system clock).
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