Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 94

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Fig. 7.16. Run Completed screen.

7.11.1 Exporting Data to a Computer

The run data file can be transferred to a portable memory device as an .ofi file, which can
be imported into Profinia software (optional) for viewing data in tabular form and in a
chromatogram. If Profinia software has not been purchased, the data file can be viewed in
any compatible spreadsheet software (for example, Microsoft Excel). To export the run
data file:

1. Insert a USB portable memory device into the USB port directly below the touch screen.

The Export Data button is initially displayed with an “X” across it, indicating that the
button is not yet functional. As soon as the system recognizes the portable memory
device, the “X” disappears and the Export Data button is available. The time required
for recognition depends on the portable memory device used and can take up to 15 sec.
(See the troubleshooting details in Appendix C if the memory device is not recognized.)

2. Touch the Export Data button. A progress bar displays indicating that the transfer is in

progress; typical transfer time is approximately 60 sec. The actual transfer time
depends on the amount of data in the run data file and the portable drive.

Upon completion of data export, you will automatically return to the Run Completed screen.

7.11.2 Performing Final Run Procedures

The toolbar at the bottom of the Run Completed screen displays the final steps to
shut down the system or prepare for a new run. Choose one of the following options:

Re-Run Method — immediately begin a new sample run with the same method

Run New Method — immediately begin performing another purification run using a
different method type, or new buffers for the run that was just performed

Short-Term Wash — if the system will not be used again on the same day, the buffer
lines and valves should be rinsed with water

Long-Term Wash — if the system will not be used in the next 2 days, it should be
rinsed with water and ethanol or other sanitizer to maintain optimum mechanical
performance and prevent bacterial growth

See section 6.10.2 for details on performing final run procedures.

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