Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 134)

9. Connect the fitting you disconnected in step 8 to the inlet of the pH flow cell.
10. Find the length of tubing supplied with the pH monitor kit. Connect one end to the outlet
fitting of the pH flow cell.
11. Connect the other end of the tubing to the center port of the outlet select valve.
12. Add a small amount of water (2 ml) to the pH flow cell.
13. Connect the pH probe’s electrical connector to the receptacle on the instrument
(Figure 11.4).
Fig. 11.4. Connecting the pH probe electrical connector.
14. Remove the plastic cap from the pH probe and install it on the pH flow cell; tighten the
black collar until it is snug. If a small amount of liquid drips from the flow cell during this
operation, wipe it off with a paper towel.
15. Replace the user access panel, four thumbscrews, and the waste bottle, and turn the
unit around so it is facing you.
16. Reconnect the waste bottle tube and power and USB cables to prepare the instrument
for operation.
17. Turn the instrument power on.
18. On the home screen, touch the Data/Utilities button, then touch the Calibration
Functions button.
19. Touch the arrow buttons to scroll through the list of calibration functions and highlight
“Calibrate pH”, then touch the Select button.
20. Follow the instructions displayed on the screen to calibrate the pH probe.
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