Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 11)

1.4 Related Consumables and Products
The following related products are available from Bio-Rad (see Appendix B for a list of
ordering information and catalog numbers):
• Detection antibodies — used for histidine (His) and glutathione S-transferase (GST)
• Bacterial/Lysis/Extraction reagent — chemical lysis buffer used to prepare sample
lysates; without the need for sonication. This reagent is not used in the instrument
• Profinia control lysate — lyophilized sample prepared with a His- and GST-tagged
protein from
E. coli
• Profinity eXact Antibody Reagent — a mouse monoclonal antibody used to detect
expression of the target protein
The following related products are available from other vendors:
• Conical tubes — 15 ml and 50 ml, for use in the sample and fraction collection positions
Note: Not all tubes made by all vendors will fit properly in the Profinia instrument; Bio-Rad
recommends the following vendor tubes: 50 ml, VWR catalog #21008-178 and #20171-029
and BD Biosciences catalog #352098; 15 ml, VWR catalog #20171-025 and BD
Biosciences catalog #352097.
1.5 About This Guide
1.5.1 Conventions
The following messages in bold are used to highlight critical information and reminders
throughout this document:
• Warning — indicates an action that can result in an irretrievable loss of sample or data
• Note — serves as a reminder, repeating relevant information from previous sections or
offering advice helpful to achieving desired results
• Important — indicates information critical to achieving optimal results
Touch-screen button commands are indicated by initial capitalization, for example: Touch
the Select button.
Screen names are indicated by initial capitalization, for example: You will proceed to the
Data/Utilities screen.
Functions located within menus on touch screens are indicated by initial capitalization and
enclosed in double quotation marks, for example: Highlight the “System Ethanol Wash”
option.
4