Bio-Rad Profinia™ Protein Purification Instrument User Manual
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Fig. 6.8. Enter Run & Sample Information screen.
Note: When two samples are selected for the method, press the View Method button in the
lower toolbar to view the method steps and the step parameters for each sample. A View
Sample button is displayed above the arrows in the View Method screen.
The following run-specific information can be entered or selected:
•
Username — optional; user-assigned name 3–15 characters in length to document the
operator of the run
•
Run name — user-designated file name for the run, up to 30 characters in length
•
(External fc) — external fraction collection option; currently not available for operation
•
Lot # tracking — optional; lot number information for the buffers, cartridges, and kits
used for the run (“BLANK” indicates no information has been entered). The word
“PRESENT” indicates that lot number information was entered. The lot number
information remains in memory as long as the instrument power remains on. When
cycling the power, the lot number information reverts to “BLANK”
The following sample-specific information can be entered or selected:
•
Sample name — optional; user-assigned name for the sample, up to 15 characters in
length (“UNTITLED” indicates no information has been entered). If you have chosen to
perform a run with two samples, you can assign a name for each sample
•
Volume — user-entered sample volume between 2 ml and 50 ml. If you have chosen
to perform a run with two samples, a volume must be entered for each sample. If the
sample volume is greater than 50 ml, see section 7 for information on program methods.
If the sample volume is less than 2 ml, diluting the sample with 1x lysis buffer to a total
volume of >10 ml is recommended to obtain optimum yield
Note: When the desalting-only method is selected, the sample volume is fixed at 2 ml for the
10 ml cartridge and 10 ml for the 50 ml cartridge. The sample volume is 20% of the cartridge
volume, the optimized volume for buffer exchange.
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