Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 74)

Fig. 6.43. Prepare System to Clean Lines, long-term wash screen.
To prepare the system for the long-term wash:
1. Place bottles of deionized water, 20% ethanol, or other sanitizer in buffer positions 1
through 7.
2. Place a bottle with 20% ethanol (or other sanitizer) in buffer position 8.
3. Place tubes containing 10 ml of deionized water, 20% ethanol, or other sanitizer in both
sample 1 and sample 2 positions.
4. Remove all cartridges and directly connect the upper and lower cartridge fittings.
5. Place the cleaning tray in the fraction collection area.
6. Touch the Start button to begin long-term storage cleaning; the cleaning progress
screen displays. Immediately below the progress bar, the current line cleaning is indicated.
When long-term cleaning is finished, a message on the touch screen appears prompting you
to cap all system lines and turn the instrument off.
Important: When the Profinia system is used in a cold environment, it is best to leave
instrument power on when not in use; the slight amount of heat generated helps control
condensation. The UV lamp automatically turns off one hour after the last separation run,
and turns back on when instrument operation resumes. If the instrument will be stored long
term in a cold environment, it is acceptable to turn the power off. When the instrument is
removed from a cold environment, the unit must be equilibrated to room temperature
before turning on the instrument. This prevents condensation from forming which could
possibly be damaging to internal components.
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