Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 196)

In order to preserve the activity of labile antibodies when using acidic elution conditions, it is
recommended that 1 M Tris-HCl, pH 8 be added to the elution fractions (1D and 2D)
containing purified antibodies when not using integrated desalting so that the final pH of the
collected fractions is adjusted to pH 7–8.
Accessories to the Profinia instrument that can be used for protein A methods include
Profinia cooling blocks, cartridge connection fittings, and external sample/buffer tubing.
Complete catalog and ordering information can be found in Section 4 of this appendix.
Table 2. Recommended formulations for buffers and solutions for 1 ml protein
A plus 10 ml desalting method
* These buffers and solutions are available as part of the Profinia desalting kit (catalog #620-0228)
† Volumes needed for the 5 ml protein A methods will be corresponding larger to accommodate the increase in cartridge capacity
and size
‡ These catalog numbers are for dry ingredients that can be used to prepare working buffer solutions
D See Section 5 for suggested recipes for these buffers
1.4 Recommended Storage Conditions
Buffers in the Profinia purification kits should be stored between 4 and 22°C. Once they are
used with the instrument, all reagents should be stored at 4°C for up to 3 months. After
insertion into the instrument, the solutions are no longer sterile. It is important to check
solutions for particulates and clarity before reusing. If there is any indication of particulates,
contamination or microbial growth, the solutions should be discarded. Affi-Prep protein A
and P6 desalting Bio-Scale Mini cartridges should be stored at 4°C in the appropriate
storage solutions listed in Table 2. Refer to the manufacturer’s recommendations for storage
of other protein A cartridges that are compatible with the Profinia system.
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