Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 169)

Table 13.1. Diameters and lengths required for Profinia system tubing connections.
System Connection System Label Tubing OD, Tubing
Inches Length,
Inches
Buffer bottle to inlet select valve port B1 to ISV 1; B2 to ISV 2; 1/8 21
B3 to ISV 3; B4 to ISV 4;
B5 to ISV 5; B6 to ISV 6;
B7 to ISV 7; B8 to ISV 8
Inlet select valve common to proportioning ISV Com to PV (NC) 1/8 8
valve NC port
Diluent bottle inlet to proportioning valve Water to PV (NO) 1/8 17
NO port
Proportioning valve common to inline filter PV (COM) to PUMP IN 1/8 12
Pump inlet tee (in pump block) to inlet check Pump in to check VLV 1/8 5
valve (below pump block), 2 each
Pump outlet tee (in pump block) to inlet check Pump out to check VLV 1/16 5
valve (above pump block), 2 each
Pump outlet to pressure sensor inlet Pump out to pressure 1/16 8
Pump wash loop (top of pump block, PWash loop 1/8 4
near sheet metal)
Pump wash outlet to waste tee (bottom PWash out to waste 1/8 8
pump block)
Inline filter outlet (above buffer bottles) to Filter out to pump in 1/8 18
pump inlet
Column valve 1 port 2 to pump wash in CV1 P2 to PWash in 1/8 8
(bottom pump block)
Pressure sensor to column valve 1 common Pressure to CV1 COM 1/16 8
(center) port
Column 1 tee to UV monitor 1 flow cell Tee to UV1 IN 1/16 8
(below UV monitor)
UV monitor 1 flow cell outlet (above UV UV1 to CV2 COM 1/16 8
monitor) to column valve 2 common
(center) port
Column valve 2 port 5 to column valve 2 tee CV2 P5 to tee 1/16 5
UV monitor 2 flow cell outlet to column UV2 out to tee 1/16 5
valve 2 tee
Column 2 tee to conductivity monitor inlet Tee to COND IN 1/16 5
(or pH flow cell inlet)
(Optional) pH flow cell to outlet selector valve pH to OSV 1/16 6
common (center) port
Sample inlet short 1/8" tube with air sensor S1 or S2 1/8 2.5
(inside electronics cabinet)
Sample inlet 1/16" tube from union (inside S1 to ISV P9; S2 to ISV P10 1/16 16
electronics cabinet) to inlet select valve
Column valve 1 port 5 to column 1 tee CV1 P6 to COL1 IN 1/16 9
Column valve 1 port 6 to column 2 Inlet CV2 P6 to COL2 IN 1/16 6
Column 1 out to column 1 tee COL1 out to TEE 1/16 27
Column 1 tee outlet to UV monitor flow cell 1 COL1 TEE to UV1 IN 1/16 27
Column 2 outlet to UV monitor 2 inlet COL2 out to UV2 IN 1/16 9
(under UV monitor)
Waste manifold to waste outlet Manifold to out 1/8 12
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