Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 72)

Re-Running a Method
To repeat the method just performed with the same buffers, touch the Re-Run Method
button in the toolbar at the bottom of the End of Run screen. You will return to the Enter
Run & Sample Information screen to repeat the Bio-Rad methods set-up and run process.
Running a New Method
Two options are available when the Run New Method button is selected upon completion
of a run: select a new method utilizing the same buffers, or select a new method that
requires a different buffer set. When the same buffers are used you will proceed directly to
the Select Method Type & Options screen to follow the procedures for starting a new run.
When different buffers are required, you can rinse the buffer lines prior to proceeding to the
home screen.
To run a new method:
1. Touch the Run New Method button in the toolbar at the bottom of the End of Run
screen. You will proceed to the Prepare System to Clean Lines screen (Figure 6.41).
Fig. 6.41. Prepare System to Clean Lines screen displays when running a new method to clean buffer
lines if a new buffer set is being used.
2. Touch the Home button in the toolbar if a new method with the same buffers will be run.
You will proceed directly to the home screen.
3. Touch the Back button to return to the End of Run screen to select a different option.
4. To select the wash buffer line cleaning procedure, follow the onscreen directions and
touch the Start button to begin the cleaning procedure. When the buffer line cleaning
procedure is complete, you will automatically proceed to the Select Method Type &
Options screen.
Cleaning the System for Short-Term Storage
If the Profinia system will not be used again on the same day, performing the short-term
wash procedure (buffer lines and pump seal wash) to maintain the system in optimum
condition is recommended. To perform a wash for short-term storage, touch the Short-Term
Wash button in the toolbar at the bottom of the End of Run screen. You will proceed to the
Prepare System to Clean Lines, short-term wash screen (Figure 6.42).
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