Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 90

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7.7 Viewing Real-Time Data With Profinia Software

To collect real-time data:

1. Connect the USB cable provided with the Profinia software package to the USB port on

the back of the instrument and to the computer.

Important: The front USB port does not output real-time data.

2. Open Profinia software on your computer by double-clicking on the desktop Profinia

software icon. Look for the connection icon in the Profinia software in the lower left
corner of the software screen (see section 12 for more information on using Profinia
software.

At this point, the run can be started and the chromatogram displays on the computer
screen.

7.8 Starting the Run

The Start Run screen (Figure 7.13) displays a summary of the sample information. It also
displays a reminder to connect the instrument to a computer running Profinia software if
you would like to view the chromatogram in real time. The Profinia system does not require
software and a computer to operate the instrument; current data points appear on the
running screen.

The lower toolbar contains buttons for viewing the methods steps and for optional manual
priming of any extra tubing attached to the sample, buffer, diluent, or waste lines to
accommodate larger containers in these ports.

Note: Touching the Home button returns the user directly to the home screen and all the
run and sample information resets, except the lot number tracking information. A saved
method remains stored in memory. An edited method that is not saved reverts to the original
settings.

Fig. 7.13. Start Run screen.

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