Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 70)

2. Place tubes containing 10 ml of deionized water in both sample 1 and sample 2 positions;
place water in both sample positions even if only one sample was used in the run.
3. Fill a buffer bottle with 20% ethanol and place it in buffer position 8.
4. Remove all fraction collection tubes from the instrument.
5. Place the cleaning tray (provided with the instrument) in the fraction collection area.
6. Touch the Start button to begin the cleaning procedure.
You will proceed to the System Cleaning in Progress screen.
6.9.2 System Cleaning in Progress
When system cleaning starts, the System Cleaning in Progress screen (Figure 6.39) displays
the four system cleaning procedures: Clean Sample Lines, Clean Fraction Lines, Clean
Buffer Lines, and System EtOH Wash. The cleaning steps that will be performed after a run
is completed are indicated by darkened circles. A checkmark adjacent to a darkened circle
indicates the cleaning step has been completed. When the cleaning process is completed,
you will proceed to the Run Completed screen where the run results are displayed.
Fig. 6.39. System Cleaning in Progress screen showing fraction line cleaning is in progress. The actual
cleaning step, indicated by a number code and brief description, is displayed below the progress bar.
Note: The Clean Buffer Lines and System EtOH Wash options are available as short-term
or long-term cleaning methods, respectively, in the Run Completed screen. All cleaning
options are also accessible via the Data/Utilities menu accessible from the home screen.
6.10 Performing End-of-Run Procedures
The Run Completed screen (Figure 6.40) is displayed upon completion of all purification run
steps, including system cleaning. Run details for each sample purified are displayed in the
run data table. The total estimated protein (yield) and protein concentration have been
calculated and are displayed for each sample.
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