Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 81)

3. Touch the arrow buttons to the right of the lot number information text box to scroll
through the list of buffers, solutions, and kit information relevant to the run. Highlight the
item for which you want to add lot number information.
Note: In this screen only, the highlighted area will automatically advance to the next line
each time lot number information is added.
4. Touch the Edit button to retrieve the alphanumeric keypad.
5. Add desired information into the keypad text line.
6. Touch OK to save input data and return to the Edit Lot Number Information screen, or
Cancel to return to the Edit Lot Number Information screen without saving changes.
7. Repeat this process for each item that requires lot number information.
8. Touch Clear All to delete all information added, or OK to accept changes and return to
the Enter Run & Sample Information screen.
Note: When lot number tracking information is entered, the word “PRESENT” is displayed.
If no lot number tracking information is entered, the word “BLANK” is displayed. All entered
run and sample information, except lot number tracking information will be cleared when
you press the Home button. The entered lot number tracking remains in temporary memory
as long as instrument power is on. When cycling power, the lot number tracking information
defaults to “BLANK”.
7.4 Editing and Saving a Program Method
The method type that was selected in the Select Method Type & Options screen serves as
a template for the program method. The sequence of the individual steps of the template
cannot be changed; however, individual parameters of the steps such as flow rate, column
volume, buffer concentration, and peak detection thresholds can be edited. See Appendix I
for method-specific planning worksheets to help guide individual method step programming.
Touching the Edit Method button in the lower toolbar of the Enter Run & Sample
Information screen displays the Method Information screen, which contains a table listing
the major steps of the run. Step parameters are edited on the Select Parameters to Edit
screen.
Important: Make sure the correct method temperature is selected for methods that include
the desalting step.
7.4.1 Editing a Program Method
To edit method parameters for your run:
1. Touch the Edit Method button in the Enter Run & Sample Information screen toolbar.
You will proceed to the Method Information screen (Figure 7.5), where the first editable
step will be highlighted.
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