Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 172)

5. Slip the lock ring over the tubing.
Note: The lock ring is tapered on the inside; the wide end of the lock ring faces the cut end
of the tubing.
6. Slip the ferrule over the tubing, with the flat end of the tubing toward the cut end of the
tube (Figure 13.25).
Fig. 13.25. Ferrule and lock ring properly slipped over the tubing.
7. Thread the nut into a ¼-28 fitting; you may wish to use the external fraction collector
connection on the back panel of the instrument. This compresses the ferrule and locks
it onto the tube (Figure 13.26).
Fig. 13.26. Ferrule locked onto the tube.
8. Repeat the steps above for the other end of the tube.
9. Using tape, label the new tubing assembly with the same words as the tubing it
replaces.
10. Install the new tubing and tighten the fittings.
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