Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 86)

• Waste bottle — left side of the touch screen and positioned at the lower left of the
instrument. On the touch screen, the waste bottle is displayed without a volume as a
reminder to make sure the waste bottle is emptied frequently
• Diluent (water) bottle — right side of the touch-screen diagram and positioned at the
lower right of the instrument. On the touch screen, the diagram is labeled with the
minimum volume of water required for the selected method run
• Sample tubes — indicated by S1 and S2 labels on the touch screen, the sample tubes
are placed beneath the buffer bottles toward the right of the instrument. The touch
screen displays one or two sample tubes with the sample volumes, depending on the
number of samples and sample volumes that have been entered by the user on the
Enter Run & Sample Information screen
Note: Each run primes the sample line(s) with approximately 1 ml of sample. To ensure the
entered sample volume is loaded on the cartridge, filling the sample tubes with 1 ml more
than the stated volume is recommended. If the exact entered volume is added to the sample
tube, the end of the sample detection, if activated, applies the complete sample to the
system. In this case, the total amount of sample added to the cartridge will be about 0.5 ml
less than the entered volume. To maximize protein yields, 10 ml is recommended sample
load.
• Fraction collection tubes — indicated on the touch screen by a set of four tubes on
the left labeled 1A through 1D for sample 1 collection, and four tubes on the right
labeled 2A through 2D for sample 2 collection. Fraction tubes are placed at the bottom
of the instrument. Each fraction tube shows the volume that will be collected in the tube.
Only the required fraction tubes are represented in the run method installation diagram.
Before beginning your run, make sure the fraction tube capacities match the volumes
indicated
Note: The fraction positions are numbered 1A through 1D from left to right, and 2A through
2D are ordered right to left. This places the purified protein in the center two positions, for
easiest access and for use with the cooling accessory.
• View Details — lower toolbar button; proceeds to a screen (Figure 7.9) that displays
the name for each of the components shown in the system setup screen. Touch OK to
return to the Install Reagents, Sample & Fraction Tube screen
Fig. 7.9. Review Buffer & Fraction Details screen displayed when the View Details button is selected.
79