Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 137)

Once the longer tubes have been installed and other standard setup procedures are
complete, the system is ready for a purification run.
11.2.3 External Fraction Collection Containers
To connect an external fraction collection container to the Profinia instrument, all that is
required is 1.6 mm ID, 0.8 mm wall silicone tubing (catalog #731-8211 or similar) sufficient
in length to connect the external container(s) to the fraction outlet port(s) on the instrument.
It is best to keep tubing length as short as possible. To use fraction containers that do not fit
in the system fraction collection tray:
1. Place fraction container(s) to be used in your purification run next to the instrument.
Note: One-sample runs require four fraction collection containers; two-sample runs require
eight fraction collection containers.
2. Locate the bottom section of tubing that is connected to each fraction outlet valve
(Figure 11.7).
Fig. 11.7. Fraction outlet valves. 1, White support tubing; 2, clear outlet tubing.
3. Slip the 1.6 mm ID tubing over the clear outlet tubing, then over the white support tubing.
4. Place the other end of the tubing into the external fraction collection container.
Note: If connecting more than one external fraction collection container for a run, it is helpful
to use stickers to label each tube with the Profinia fraction tube position number marked on
the instrument.
Once the longer tubes are installed and other standard setup procedures are complete, the
system is ready for a purification run.
11.3 Profinia Cooling Accessory
The Profinia cooling accessory (Figure 11.8) is designed for users that need to keep protein
sample and fraction temperatures below 4°C, but don’t have access to a cold room. This
accessory holds either two 15 ml, two 50 ml, or one of each size conical tubes. The cooling
accessory cools the two samples or the two purified protein fractions.
130
1
2